Engineering & Histidine Switching

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2026-03-19 07:47:40

Question: What is the primary protein engineering strategy used to create pH-sensitive binding affinity? Answer: The most common approach involves "Histidine switching," where specific residues in the complementarity-determining regions (CDRs) are mutated to Histidine. Because the imidazole side chain of Histidine becomes protonated and positively charged at acidic pH, it can trigger...

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